Peer-Reviewed Original Research

Wu D, Tripathi D, Manhas A, Noishiki C, Liu L, Wu CA, Venkateshappa R, Zhang H, Ren L, Thomas D, Nalbandian M, Limbu L, DaValle CC, Rangan ES, Nallamshetty S, Boyd JH, Malik SB, Woo YJ, Sayed D, Sallam K, Blau HM, Wu JC, Sayed N*. RUNX1-driven endothelial-to-mesenchymal transition contributes to remodelling in LMNA cardiomyopathy. European Heart Journal. 2026. IN PRESS PubMed ↗

Wu MA, Wu CA, Zhao SR, Sun J, Flores-Banuelos AG, Walther P, Wheeler M, Sayed N*. Characterization of induced pluripotent stem cell lines from patients of African American ancestry. Stem Cell Res. 2026 Jun 29;95:104053. PubMed ↗

Wu CA, Wu MA, Zhao SR, Sun J, Flores-Banuelos AG, Walther P, Wheeler M, Sayed N*. Generation of two induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying MYBPC3 mutations. Stem Cell Res. 2026 Apr 1;94:103982. PubMed ↗

Liu L, Manhas A, Noishiki C, Wu D, Tripathi D, Turbes N, Sallam K, Lee JT, Sayed N*. Generation of an induced pluripotent stem cell line from a patient with Loeys-Dietz syndrome. Stem Cell Res. 2026 Apr 1;94:103981. PubMed ↗

Thomas D, Manhas A, Liu Y, Venkateshappa R, Belbachir N, Zhao S, Juguilon C, Chen IY, Moslehi, Sayed N*, Wu JC. Profiling Immune-Independent Response to Immune Checkpoint Inhibitors on iPSC-Derived Cardiomyocytes, Organoids, and Mouse Models. Circulation. 2026 Jan 13;153(2):132-135. PubMed ↗

Liu L, Wu D, Tsao PS, Leeper NJ, Sayed N*. Harnessing iPSCs, 3D Organoids, and Multi-Omics to Model Rare Vascular Diseases: Emerging New Approach Methodologies. Vascular Medicine. 2026 Jan 7:1358863X251394285. PubMed ↗

Manhas A, Liu Y, Noishiki C, Wu D, Tripathi D, Mirza S, Thomas D, Liu L, Guha A, Nguyen PK, Chen IY, Chitalia V, Cheng PC, Sayed D, Telli ML, Sallam K, Wu JC, Sayed N*. Multiscale profiling of tyrosine kinase inhibitor cardiotoxicity reveals mechanosensitive ion channel PIEZO1 as cardioprotective. Science Translational Medicine. 2025 Dec 17;17(829):eadv9403. PubMed ↗

Noishiki C, Manhas A, Adkar SS, Tripathi D, Wu D, Sadat S, Liu L, Sallam K, Leeper NJ, Fukaya E, Sayed N*. Generation of an induced pluripotent stem cell line from a patient with Varicose veins. Stem Cell Res. 2025 Oct 9;89:103850. PubMed ↗

Thomas D, Yang PC, Wu JC, Sayed N*. Decoding Long COVID-Associated Cardiovascular Dysfunction: Mechanisms, Models, and New Approach Methodologies. Journal of Molecular and Cellular Cardiology. 2025 Sep 30:209:37-50. PubMed ↗

Lotfollahzadeh S, Paul H, Bonifacio J, Almiron R, Hockestra I, Prezkop K, Yamamoto T, Piqueras MC, Yin W, Malikova M, Siracuse JJ, Belghasem M, Cabral H, Sayed N*, Chitalia V. Kidney360. 2025 Oct 13. PubMed ↗

Thakkar C, Alikunju S, Venkatasubramanian A, D'Mello D, Abbas H, Yang Z, Andreas I, Sayed N, Abdellatif M, Sayed D. Constitutive expression of cardiomyocyte Klf9 precipitates metabolic dysfunction and spontaneous cardiomyopathy. Cell Signal. 2025 Sep 22;136:112146. PubMed ↗

Elezaby A, Dexheimer R, Wu D, Chan SY, Chen IY, Sayed N*, Sallam K. Immunosuppression Drugs Exhibit Differential Effects on Endothelial Cell Function. J Vasc Res. 2025 Sep 11:1-22. PubMed ↗

Mondéjar-Parreño G, Zhao SR, Cao X, Liu Y, Yang JY, Jahng JWS, Leitz J, Wu D, Sayed N*, Jalife J, Wu JC. Investigating the Risk of Arrhythmogenesis Associated with Fentanyl Abuse Using Human and Mouse Cardiomyocytes. Circulation. 2025 Aug 26;152(8):563-566. PubMed ↗

Huynh DTN, Noishiki C, Lai J, Yarahmadi P, Le T, Tripathi D, Tacco IR, Manhas A, Sallam K, Chen IY, Sayed N*, Nguyen PK. Generation of an induced pluripotent stem cell line from a malignant melanoma patient who developed the immune checkpoint inhibitor-related myasthenia gravis, myositis, and myocarditis overlap syndrome. Stem Cell Res. 2025 Aug 6;87:103797. PubMed ↗

Sayed N*. Are We There Yet? JAMA. 2025 Aug 5;334(5):393-394. PubMed ↗

Kort EJ, Sayed N*, Liu C, Mondéjar-Parreño G, Forsberg J, Eugster E, Wu SM, Wu JC, Jovinge S. Olmesartan restores LMNA function in haploinsufficient cardiomyocytes. Circulation. 2025 Apr 1. Online ahead of print.. PubMed ↗

Ross Tacco I, Olshausen J, Chan TY, Turbes N, Hung MY, Yeh CT, Nguyen PK, Sallam K, Sayed N*, Chen IY. Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm. Stem Cell Res. 2025 Feb;82:103644. Epub 2024 Dec 24.. PubMed ↗

Wu D, Manhas A, Noishiki C, Tripathi D, Liu L, Turbes N, Thomas D, Sallam K, Lee JT, Sayed N*. Generation of induced pluripotent stem cell line from a patient with long COVID. Stem Cell Res. 2025 Jan 4;83:103652. Online ahead of print.. PubMed ↗

Liu C, Shen M, Liu Y, Manhas A, Zhao SR, Zhang M, Belbachir N, Ren L, Zhang JZ, Caudal A, Nishiga M, Thomas D, Zhang A, Yang H, Zhou Y, Ameen M, Sayed N*, Rhee JW, Qi LS, Wu JC. Druggable genome CRISPRi/a screens in iPSC-derived cardiomyocytes identify therapeutic targets for doxorubicin-induced cardiotoxicity. Cell Stem Cell 2024 Dec 5;31(12):1760-1776.e9. Epub 2024 Nov 7.. PubMed ↗

Thomas D, Noishiki C, Gaddam S, Wu D, Manhas A, Liu Y, Tripathi D, Kathale N, Adkar SS, Garhyan J, Liu C, Xu B, Ross EG, Dalman RL, Wang KC, Oro AE, Sallam K, Lee JT, Wu JC, Sayed N*. CCL2-mediated Endothelial Injury Drives Cardiac Dysfunction in Long COVID. Nat Cardiovasc Res. 2024 Oct;3(10):1249-1265. Epub 2024 Oct 14.. PubMed ↗

Wu Y, Sun T, Medina P, Narasimhan P, Stevenson DK, Von Versen-Höynck F, Armstrong J, Wu JC, Sayed N*, Winn VD. A novel stem cell model to study preeclampsia endothelial dysfunction. Reprod Sci. 2024 Oct;31(10):2993-3003. Epub 2024 Aug 23.. PubMed ↗

Dexheimer R, Manhas A, Wu D, Tripathi D, Yu Chan S, Li J, Yu R, Sayed N*, Wu JC, Sallam K. Generation of two iPSC lines from dilated cardiomyopathy patients with pathogenic variants in the SCN5A gene. Stem Cell Res. 2024 Oct;80:103498. Epub 2024 Jul 20.. PubMed ↗

Manhas A, Tripathi D, Thomas D, Sayed N*. Cardiovascular toxicity in cancer therapy: protecting the heart while combating cancer. Curr Cardiol Rep. 2024 Sep;26(9):953-971. Epub 2024 Jul 23.. PubMed ↗

Manhas A, Tripathi D, Noishiki C, Wu D, Liu L, Sallam K, Lee JT, Fukaya E, Sayed N*. Generation of two iPSC lines from vascular Ehlers-Danlos Syndrome (vEDS) patients carrying a missense mutation in COL3A1 gene. Stem Cell Res. 2024 Sep;79:103485. Epub 2024 Jun 25.. PubMed ↗

Wu F, Du H, Overbey E, Kim JK, Makhijani P, Martin N, Lerner CA, Nguyen K, Baechle J, Valentino TR, Fuentealba M, Bartleson JM, Halaweh H, Winer S, Meydan C, Garrett-Bakelman F, Sayed N*, Melov S, Muratani M, Gerencser AA, Kasler HG, Beheshti A, Mason CE, Furman D, Winer DA. Single-cell analysis identifies conserved features of immune dysfunction in simulated microgravity and spaceflight. Nat Commun. June 2024. 15, 479. PubMed ↗

Nain P, Seth L, Patel V, Jiang S, Gopu G, Singh R, Stabellini N, Reddy R, Weintraub NL, Harris RA, Cullen J, Agarwal N, Moore JX, Sayed N*, Avirup Guha. Understanding Cardiovascular Risk in Prostate Cancer: Role of Disparities, Diabetes, and Aging. Curr Treat Options Cardio Med. April 2024. 26, 93–109. PubMed ↗

Tripathi D, Manhas A, Noishiki C, Wu D, Adkar S, Sallam K, Fukaya E, Leeper NJ, Sayed N*. Generation of induced pluripotent stem cell line from a patient suffering from arterial calcification due to deficiency of CD73 (ACDC). Stem Cell Res. 2024 Mar;75:103285. Epub 2023 Dec 19.. PubMed ↗

Thomas D, Manhas A, Sayed N*. Harnessing iPSCs to Dissect Causality in Anthracycline-Induced Cardiotoxicity: All That Fits Are Not Hits. JACC CardioOncol. 2024 Feb 20;6(1):51-54.. PubMed ↗

Alsaigh T, Dhaliwal G, Fukaya E, Leeper NJ, Sayed N*. An Alternate Explanation. N Engl J Med. 2023 Apr 6;388(14):1318-1324. Erratum in: N Engl J Med. 2023 Sep 21;389(12):1156.. PubMed ↗

Liu C, Shen M, Tan WL, Chen IY, Liu Y, Yu X, Yang H, Zhang A, Liu Y, Zhao MT, Ameen M, Zhang M, Gross ER, Qi LS, Sayed N*, Wu JC. Statins improve endothelial function via suppression of epigenetic-driven EndMT. Nat Cardiovasc Res. 2023 May;2(5):467-485. Epub 2023 May 8.. PubMed ↗

Sayegh N, Yirerong J, Agarwal N, Addison D, Fradley M, Cortes J, Weintraub NL, Sayed N*, Raval G, Guha A. Cardiovascular Toxicities Associated with Tyrosine Kinase Inhibitors. Curr Cardiol Rep. 2023 Apr;25(4):269-280. Epub 2023 Feb 16.. PubMed ↗

Sun T, Cruz G, Mousavi N, Marić I, Brewer A, Wong RJ, Aghaeepour N, Sayed N*, Wu JC, Stevenson DK, Leonard SA, Gymrek M, Winn VD. HMOX1 Genetic Polymorphisms Display Ancestral Diversity and May be Linked to Hypertensive Disorders in Pregnancy. Reprod Sci. 2022 Dec;29(12):3465-3476. 022-01001-1. Epub 2022 Jun 13.. PubMed ↗

Ramachandra AB, Wang H, Wnorowski A, Schwarz EL, Pickering J, Heiler JC, Lucian HJ, Hironaka CE, Tran NA, Liu Y, Khan MO, Obafemi O, Tada Y, Kahn AM, Sayed N*, Wu JC, Humphrey JD, Boyd JH, Marsden AL. Biodegradable external wrapping promotes favorable adaptation in an ovine vein graft model. Acta Biomater. 2022 Oct 1;151:414-425. Epub 2022 Aug 20.. PubMed ↗

Wei TT, Chandy M, Nishiga M, Zhang A, Kumar KK, Thomas D, Manhas A, Rhee S, Justesen JM, Chen IY, Wo HT, Khanamiri S, Yang JY, Seidl FJ, Burns NZ, Liu C, Sayed N*, Shie JJ, Yeh CF, Yang KC, Lau E, Lynch KL, Rivas M, Kobilka BK, Wu JC. Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation. Cell. 2022 Jun 23;185(13):2387-2389.. PubMed ↗

Sallam K, Thomas D, Gaddam S, Lopez N, Beck A, Beach L, Rogers AJ, Zhang H, Chen IY, Ameen M, Hiesinger W, Teuteberg JJ, Rhee JW, Wang KC, Sayed N*, Wu JC. Modeling Effects of Immunosuppressive Drugs on Human Hearts Using Induced Pluripotent Stem Cell-Derived Cardiac Organoids and Single-Cell RNA Sequencing. Circulation. 2022 Apr 26;145(17):1367-1369. Epub 2022 Apr 25.. PubMed ↗

Thomas D, de Jesus Perez VA, Sayed N*. An evidence appraisal of heart organoids in a dish and commensurability to human heart development in vivo. BMC Cardiovasc Disord. 2022 Mar 22;22(1):122. 02543-7.. PubMed ↗

Alikunju S, Niranjan N, Mohsin M, Sayed N*, Sayed D. G3bp1-microRNA-1 axis regulates cardiomyocyte hypertrophy. Cell Signal. 2022 Mar;91:110245. Epub 2022 Jan 10.. PubMed ↗

Chen IY, Vedula V, Malik SB, Liang T, Chang AY, Chung KS, Sayed N*, Tsao PS, Giacomini JC, Marsden AL, Wu JC. Preoperative computed tomography angiography reveals leaflet-specific calcification and excursion patterns in aortic stenosis. Circ Cardiovasc Imaging. 2021 Dec;14(12):1122-1132. Epub 2021 Dec 17.. PubMed ↗

Alvi SB, Ahmed S, Sridharan D, Naseer Z, Pracha N, Wang H, Boudoulas KD, Zhu W, Sayed* N, Khan M. De novo Drug Delivery Modalities for Treating Damaged Hearts: Current Challenges and Emerging Solutions. Front Cardiovasc Med. 2021 Sep 28;8:742315.. PubMed ↗

Sayed N, Huang Y, Nguyen K, Krejciova-Rajaniemi Z, Grawe AP, Gao T, Tibshirani R, Hastie T, Alpert A, Cui L, Kuznetsova T, Rosenberg-Hasson Y, Ostan R, Monti D, Lehallier B, Shen-Orr SS, Maecker HT, Dekker CL, Wyss- Coray T, Franceschi C, Jojic V, Haddad F, Montoya JG, Wu JC, Davis MM, Furman D. An inflammatory aging clock (iAge) based on deep learning tracks multimorbidity, immunosenescence, frailty and cardiovascular aging. Nat Aging. 2021 Jul;1:598-615. Epub 2021 Jul 12. Erratum in: Nat Aging. 2021 Aug;1(8):748.. PubMed ↗

Liu C, Medina P, Thomas D, Chen IY, Sallam K, Sayed D, Sayed N*. A protocol for transdifferentiation of human fibroblasts to endothelial cells via activation of innate immunity. STAR Protoc. 2021 Jun 5;2(2):100556.. PubMed ↗

Tran MV, Marceau E, Liu Y, Sallam K, Medina P, Liu C, Sayed N*, Muller MD, Liang DH, Chen IY. Coronary artery vasospasm requiring cardiac autotransplantation yet controlled with tobacco. JACC Case Rep. 2021 May 26;3(9):1177-1181.. PubMed ↗

Shah PP, Lv W, Rhoades JH, Poleshko A, Abbey D, Caporizzo M, Linares-Saldana R, Heffler J, Sayed N*, Thomas D, Wang Q, Stanton LJ, Bedi K, Morley MP, Cappola TP, Owens AT, Margulies KB, Frank DB, Wu JC, Rader DJ, Yang W, Prosser BL, Musunuru K, Jain R. Pathogenic LMNA variants disrupt cardiac lamina-chromatin interactions and de-repress alternative fate genes. Cell Stem Cell. 2021 May 6;28(5):938-954.e9. Epub 2021 Feb 1.. PubMed ↗

Huang X, Shen W, Veizades S, Liang G, Sayed N*, Nguyen PK. Single-cell transcriptional profiling reveals sex and age diversity of gene expression in mouse endothelial cells. 2021 Feb 17;12:590377.. PubMed ↗

Thomas D, Shenoy S, Sayed N*. Building multi-dimensional induced pluripotent stem cells-based model platforms to assess cardiotoxicity in cancer therapies. Front Pharmacol. 2021 Feb 18;12:607364.. PubMed ↗

Tse G, Li KHC, Cheung CKY, Letsas KP, Bhardwaj A, Sawant AC, Liu T, Yan GX, Zhang H, Jeevaratnam K, Sayed N*, Cheng SH, Wong WT. Arrhythmogenic mechanisms in hypokalaemia: insights from pre-clinical models. Front Cardiovasc Med. 2021 Feb 3;8:620539.. PubMed ↗

Sridharan D, Palaniappan A, Blackstone BN, Dougherty JA, Kumar N, Seshagiri PB, Sayed N*, Powell HM, Khan M. In situ differentiation of human-induced pluripotent stem cells into functional cardiomyocytes on a coaxial PCL- gelatin nanofibrous scaffold. Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111354. Epub 2020 Aug 11.. PubMed ↗

Liu C, Ameen M, Himmati S, Thomas D, Sayed N*. Generation of human iPSCs by protein reprogramming and stimulation of TLR3 signaling. Methods Mol Biol. 2021;2239:153-162.. PubMed ↗

Sayed N*, Liu C, Ameen M, Himmati F, Zhang JZ, Khanamiri S, Moonen JR, Wnorowski A, Cheng L, Rhee JW, Gaddam S, Wang KC, Sallam K, Boyd JH, Woo JY, Rabinovitch M, Wu JC. Clinical trial in a dish using iPSCs shows lovastatin improves endothelial dysfunction and cellular crosstalk in LMNA cardiomyopathy. Sci Transl Med. 2020 Jul 29;12(554):eaax9276.. PubMed ↗

Rhee JW, Yi H, Thomas D, Lam CK, Belbachir N, Tian L, Qin X, Malisa J, Lau E, Paik DT, Kim Y, Choi BS, Sayed N*, Sallam K, Liao R, Wu JC. Modeling secondary iron-overload cardiomyopathy with human induced pluripotent stem cell-derived cardiomyocytes. Cell Rep. 2020 Jul 14;32(2):107886.. PubMed ↗

Tian L, Oikonomopoulos A, Liu C, Kitani T, Shrestha R, Chen CL, Ong SG, Smeets M, Karakikes I, Sayed N*, Wu JC. Molecular signatures of beneficial class effects of statins on human induced pluripotent stem cell-derived cardiomyocytes. Circulation. 2020 Apr 7;141(14):1208-1210. Epub 2020 Apr 6.. PubMed ↗

Liu C, Ruan H, Himmati F, Zhao MT, Chen CC, Makar M, Chen IY, Sallam K, Mocarski ES, Sayed D, Sayed N*. HIF1α regulates early metabolic changes due to activation of innate immunity in nuclear reprogramming. Stem Cell Reports. 14(2):192-200. February 2020. PubMed ↗

Wnorowski A, Sharma A, Chen H, Wu H, Shao NY, Sayed N*, Countryman S, Stodieck L, Rubins KH, Wu SM, Lee PU, Wu JC. Effects of spaceflight on human induced pluripotent stem cell-derived cardiomyocyte structure and function. Stem Cell Reports. 2019 Dec 10;13(6):960-969. Epub 2019 Nov 7.. PubMed ↗

Sass G, Tsamo AT, Chounda GM, Nangmo PK, Sayed N*, Bozzi A, Wu JC, Nkengfack AE, Stevens DA. Vismione B interferes with Trypanosoma cruzi infection of vero cells and human stem cell-derived cardiomyocytes. Am J Trop Med Hyg. 2019 Dec;101(6):1359-1368.. PubMed ↗

Sass G, Roy M, Lydia-Marie J, Bozzi A, Sayed N, Wu JC, Stevens DA. A combination of itraconazole and amiodarone is highly effective against Trypanosoma cruzi infection of human stem cell-derived cardiomyocytes. Am J Trop Med Hyg. 2019 Aug;101(2):383-391.. PubMed ↗

Yang H, Qin X, Wang H, Zhao X, Liu Y, Wo HT, Liu C, Nishiga M, Chen H, Ge J, Sayed N, Abilez OJ, Ding D, Heilshorn SC, Li K. An in vivo miRNA delivery system for restoring infarcted myocardium. ACS Nano. June 2019. 9(13), p.9880-9894. PubMed ↗

Bozzi A, Sayed N, Matsa E, Sass G, Neofytou E, Clemons KV, Correa-Oliveira R, Stevens DA, Wu JC. Using human induced pluripotent stem cell-derived cardiomyocytes as a model to study trypanosoma cruzi infection. Stem Cell Reports. 2019 Jun 11;12(6):1232-1241. Epub 2019 May 16.. PubMed ↗

Stack P, Moslehi J, Sayed N*, Wu JC. Chemotherapy-induced cardiomyopathy: Can human induced pluripotent stem cell modeling help prevent it? Eur Heart J. 2019 Jun 7;40(22):1764-1770.. PubMed ↗

Zhang JZ, Termglinchan V, Shao NY, Itzhaki I, Liu C...Kitani T, Wu H, Lam CK, Kodo K, Sayed N, Blau HM, Wu JC. A human iPSC double-reporter system enables purification of cardiac lineage subpopulations with distinct function and drug response profiles. Cell Stem Cell. 2019 May 2;24(5):802-811.e5. Epub 2019 Mar 14.. PubMed ↗

Kitani T, Ong SG, Lam CK, Rhee JW, Zhang JZ, Oikonomopoulos A, Ma N, Tian L, Lee J, Telli ML, Witteles RM, Sharma A, Sayed N*, Wu JC. Human induced pluripotent stem cell model of trastuzumab-induced cardiac dysfunction in patients with breast cancer. Circulation. 2019 May 21;139(21):2451-2465.. PubMed ↗

Severinova E, Alikunju S, Deng W, Dhawan P, Sayed N, Sayed D. Glucocorticoid receptor-binding and transcriptome signature in cardiomyocytes. J Am Heart Assoc. 2019 Mar 19;8(6):e011484.. PubMed ↗

Sayed N*, Ameen M, Wu JC. Personalized medicine in cardio-oncology: the role of induced pluripotent stem cell. Cardiovasc Res. 2019 Apr 15;115(5):949-959.. PubMed ↗

Khanamiri S, Rhee JW, Paik DT, Chen IY, Liu C, Sayed N*. Marked vascular dysfunction in a case of peripartum cardiomyopathy. J Vasc Res. February 2019;56(1):11-15. Epub 2019 Feb 14.. PubMed ↗